2 edition of Mutants of Chinese hamster cells in vitro found in the catalog.
Mutants of Chinese hamster cells in vitro
Michael William McBurney
1974 in [Toronto] .
Written in English
|Contributions||Toronto, Ont. University.|
|The Physical Object|
|Pagination||vi, 188 leaves.|
|Number of Pages||188|
Ioannou, and G. Soares, L. Grace and G. Epub Feb Such lines are said to be transformed, and are often used in research as a standardized cell line. Figure 2 Differences in expression of two AdK isoforms in various tissues and cell lines.
Reed CK. Our results show that these mutants exhibit interesting differences in their cross-resistance pattern towards the N- and C- Ado analogs and also in the expression profiles of the two AdK isoforms. Leonova and G. A much reduced ability to recover control rates of semiconservative DNA synthesis after UV irradiation was observed in the repair-deficient B cell line.
Centrifugal elutriation. Epub Dec Lee AC. Hence, to gain further insights into the cellular functions of AdK, in the present work we report the isolation and characterization of mutants resistant to Ado analogs from V79 and other CH cell lines. Fong C. This sequence region encodes for all amino acids that are distinctive of the AdK-S isoform Figure 1lower part.
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View at: Google Scholar D. The biochemical nature of the ionizing radiation sensitivity of Mutants of Chinese hamster cells in vitro book and irs2 was also investigated. Such lines are said to be transformed, and are often used in research as a standardized cell line.
An ultraviolet UV light-sensitive mutant of Chinese hamster ovary cells CHO has been isolated and characterized with respect to a number of post-irradiation responses. Pollock SJ. Domurat F. Brumshtein, S. Morganti, M. Abeysinghe H. Abraham GN. Hence, to gain further insights into the cellular functions of AdK, in the present work we report the isolation and characterization of mutants resistant to Ado analogs from V79 and other CH cell lines.
AdK, in addition to its central role in purine salvage and ATP catabolism, also plays a critical role in the maintenance of methylation reactions. These isoforms differ from each other only in their N-termini. Here, it should be taken into account the fact that GCR is a membrane-associated glycoprotein secreted at low level or rather not secreted under natural conditions [ 37 ].
Sheu TJ. Takasaki, G. Yeo et al. Currently, high-throughput methods to screen high-producer cells have been developed [ 4849 ]; including automated systems such as colony picking and the Cello systems [ 50 ]. All three of these mutants i. Studies with the recombinant AdK-L and AdK-S proteins have revealed no differences in their biochemical or kinetic properties [ 23 ] unpublished results.
However, there is no information available regarding the unique functional aspects or regulation of these isoforms. Further, as reported in our earlier work [ 34 ], the FomR-4 mutant was highly resistant to FoA but it displayed negligible resistance to tubercidin.
Pollock S. The GCR cDNA was cloned into the pED dicistronic expression vector, which is commonly used to obtain high-expression levels of heterologous proteins in mammalian cells [ 45 — 47 ], and positive clones were isolated and amplified with increasing MTX concentration up to nM MTX.
Steer, N. The resistance levels of these mutants to these analogs is similar to that for the toyocamycin and tubercidin resistant mutant of CHO cells including the ToyR-4 and DrToyR isolated previously [ 2941 ]. Rasmussen, G. Richards, J. MTX selection of amplificants has been considered a major bottleneck in the production of biopharmaceuticals because many rounds are required to obtain cells with high-gene copy numbers [ 2848 ].
Ioannou, and G. Halterman M. References 1. Felch ME.today are made in Chinese hamster ovary (CHO) cells (Jayapal et al. ). The popularity of CHO cells can be attributed to the following reasons.
Firstly, since CHO cells have been dem-onstrated as safe hosts for the past two decades, it may be easier to obtain approval to market the therapeutic proteins from regulatory agencies like the FDA. thermotolerance-deficient mutants of Chinese hamster ovary cells Callaghan, J.
M., Toh, B.H., Pettitt, J.
M., Humphris, D. C. and Gleeson, P. A. Poly-iV-acetyllactosamine-specific tomato lectin interacts with gastric parietal cells. Identification of a tomato-lectin binding A/X 10 r membrane glycoprotein of tubulovesicles Nov 22, · Construction of BAC-based physical map for Chinese hamster ovary cells.
A CHO genomic BAC library consisted ofclones was constructed in a previous study .Three hundred BAC clones were randomly selected from this library and mapped onto each chromosome of CHO DG44 cells by tjarrodbonta.com by: 1.Methods in Cell Biology.
Academic Press, Jan 13, - Science - pages. 0 Reviews. Methods in Cell Biology. Preview this book.Jan 01, · The responses of irs1 and irs2 have some similarities to those of cells from persons download pdf the cancer-prone syndrome ataxia telangiectasia (AT), but the dissimilarities between both irs mutants and AT cell lines suggest that the primary defects are distinct.", T1 - Biochemical and genetic analysis of the Chinese hamster mutants irs1 and irs2 Cited by: CHO ebook have a culture history of more than 50 years, since they were established from the ovary of a Chinese Hamster and became “immortal” in the laboratory of Dr.
Theodore Puck in the late s [2,3]. For the sake of simplicity, we call the first immortalized Cited by: